CD256, TALL2, TALL-2, TNFSF13, APRIL, APRILFLJ57090, TRDL1, TRDL-1, ZTNF2, 2310026N09Rik
Recombinant Biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag, Flag tag and Avi tag at the N-Terminus.It contains Lys112-Leu250 trimer design.
Molekulargewicht:
The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 56-65 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Lys112-Leu250
Target-Kategorie:
APRIL/TNFSF13
Biotinylated Cynomolgus APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus APRIL Trimer is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
Loaded Biotinylated Cynomolgus APRIL (Trimer), His Tag on Anti-His-Biosensor can bind Human TACI, hFc Tag with an affinity constant of 4.55 nM as determined in BLI assay (Gator Prime).
Loaded Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag on Anti-His-Biosensor can bind Cynomolgus BCMA, hFc Tag with an affinity constant of 1.87 nM as determined in BLI assay (Gator Prime).
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