Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Thr141-Leu285 trimer design.
Molekulargewicht:
The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Thr141-Leu285
Target-Kategorie:
BAFF/TNFSF13B/CD257
Human BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BAFF Trimer is greater than 95% as determined by SEC-HPLC.
Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200).
Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test).
Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 1.53 nM as determined in BLI assay (Gator Prime).
Loaded Biotinylated Human BAFFR, His-Avi Tag on Streptavidin-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator Prime).
Immobilized Human BAFF Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BAFF Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
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