Human PD-1/PDCD1 Protein (PD1-HM201)

Produktübersicht

• Artikelname: Human PD-1/PDCD1 Protein (PD1-HM201)
• Artikelnummer: KAB-PD1-HM201
• Hersteller Artikelnummer: PD1-HM201
• Alternativnummer: KAB-PD1-HM201-100UG, KAB-PD1-HM201-500UG
• Hersteller: KactusBio
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Human
• Alternative Synonym: PDCD1, PD1, CD279, SLEB2, PD-1

Produktbeschreibung

Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu25-Gln167.

Produkteigenschaften

• Molekulargewicht: The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: Q15116
• Puffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Reinheit: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Formulierung: Lyophilized
• Sequenz: Leu25-Gln167
• Target-Kategorie: PD-1/PDCD1

Bilder

Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%.

Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200).

The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.

Immobilized Human PD-1, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA.

Immobilized Human PD-L1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test).

Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L1, His Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).

Serial dilutions of Anti-PD-1 Antibody were added into Human PD-1, hFc Tag: Biotinylated Human PD-L1, His Avi Tag binding reaction. The half maximal inhibitiory concentration (IC50) is 0.22ug/ml.