Non-biotinylated Human VEGF165 Protein (VEG-HM465)

Produktübersicht

• Artikelname: Non-biotinylated Human VEGF165 Protein (VEG-HM465)
• Artikelnummer: KAB-VEG-HM465
• Hersteller Artikelnummer: VEG-HM465
• Alternativnummer: KAB-VEG-HM465-100UG, KAB-VEG-HM465-500UG
• Hersteller: KactusBio
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Human
• Alternative Synonym: VEGF, VEGFA, MVCD1, VAS, VEGFMGC70609, VPF, RP1-261G23.1, MGC70609,VEGF-165

Produktbeschreibung

Recombinant Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.

Produkteigenschaften

• Molekulargewicht: The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P15692
• Puffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Reinheit: > 95% as determined by Bis-Tris PAGE
• Formulierung: Lyophilized
• Sequenz: Ala27-Arg191
• Target-Kategorie: VEGF165

Bilder

Human VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Immobilized Human VEGF165 at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA.

Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.

Immobilized Human VEGF165, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA.

Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R2, hFc Tag with an affinity constant of 2.34 nM as determined in BLI assay (Gator Prime).

Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R1, hFc Tag with an affinity constant of 0.94 nM as determined in BLI assay (Gator Prime).

Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Anti-VEGF165 Antibody, hFc-Avi Tag with an affinity constant of 31.50 pM as determined in BLI assay (Gator Prime).

Immobilized Human VEGF165, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody2, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.