Anti-LC3 (Human) mAb, Clone: [4E12], Mouse, Monoclonal
Artikelnummer:
MBL-M152-3
- Bilder (4)
Artikelname: | Anti-LC3 (Human) mAb, Clone: [4E12], Mouse, Monoclonal |
Artikelnummer: | MBL-M152-3 |
Hersteller Artikelnummer: | M152-3 |
Alternativnummer: | MBL-M152-3 |
Hersteller: | MBL |
Wirt: | Mouse |
Kategorie: | Antikörper |
Applikation: | FC, ICC, IP, WB |
Spezies Reaktivität: | Hamster, Human, Mouse, Rat |
Immunogen: | Recombinant human LC3 (MAP1LC3B: 1-120 a.a.) |
Alternative Synonym: | 1010001C15Rik, 1010001H21Rik, 4922501H04Rik, Atg8, ATG8E, ATG8F, autophagy-related protein LC3 A, autophagy-related protein LC3 B, autophagy-related ubiquitin-like modifier LC3 A, autophagy-related ubiquitin-like modifier LC3 B, LC3, LC3A, LC3b, LC3-I, LC3-II, MAP1 light chain 3-like protein 1, MAP1 light chain 3-like protein 2, MAP1A/1B light chain 3 A, MAP1A/1B light chain 3 B, MAP1A/1BLC3, MAP1A/MAP1B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1BLC3, Map1lc3, MAP1LC3B-a, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A, microtubule-associated proteins 1A/1B light chain 3B, Mpl3, zbs559 |
Anti-LC3 (Human) mAb, IgG1 kappa, Clone: 4E12, Mouse, Monoclonal |
LC3, a mammalian homologue of yeast ATG8, is a soluble protein that is distributed ubiquitously in mammalian tissues and cultured cells. LC3 has been studied most extensively and is frequently used as an autophagy marker in mammals. This LC3 antibody is validated for multiple applications (WB, ICC, IP, FCM) and has over 25 citations. This is a monoclonal antibody of 200 μg targeting LC3 for FCM, ICC, IP, WB. |
Klonalität: | Monoclonal |
Konzentration: | 2 mg/mL |
Klon-Bezeichnung: | [4E12] |
Isotyp: | IgG1 kappa |
NCBI: | 81631 |
Puffer: | 200 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
Quelle: | This antibody was purified from hybridoma (clone 4E12) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)]. |
Formulierung: | 200 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
Lagerung: | -20°C |
Formel: | 200 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
Target-Kategorie: | LC3 |
Kommentar: | For Research Use Only. Not for use in diagnostic procedures. |
Application Verdünnung: | WB: 5 µg/mL (chemiluminescence detection system). IP: 5 µg/300 µL of cell extract from 1x107 cells. FCM: 40 µg/mL (final concentration). ICC: 40 µg/mL. Other: Immuno-EM: 20 µg/mL |