CTLA4 Antibody [1E6], IgG1, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: CTLA4 Antibody [1E6], IgG1, Unconjugated, Mouse, Monoclonal
• Artikelnummer: PRS-RF16011
• Hersteller Artikelnummer: RF16011
• Alternativnummer: PRS-RF16011-0.02,PRS-RF16011-0.1
• Hersteller: ProSci
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IF, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
• Konjugation: Unconjugated
• Alternative Synonym: CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/mL
• Klon-Bezeichnung: [1E6]
• Molekulargewicht: Predicted: 25 kDa Observed: 32 kDa
• Isotyp: IgG1
• NCBI: 1493
• UniProt: P16410
• Puffer: CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 0.125 - 0.25 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Bilder

Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 &956,g/mL. Red: CTLA4 Antibody [1E6] (RF16011) Blue: DAPI staining

Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 &956,g/mL. Red: CTLA4 Antibody [1E6] (RF16011) Blue: DAPI staining

Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 &956,g/mL. Red: CTLA4 Antibody [1E6] (RF16011) Blue: DAPI staining

Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 &956,g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 &956,g/mL.

Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 &956,g/mL.

Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 &956,g/mL.

Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with RF16011, RF16012, and RF16013 antibodies at decreasing concentrations.

A sandwich ELISA was performed using the anti-CTLA-4 mAbs RF16011, RF16012, RF16013, as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies.

Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.125 and 0.25 &956,g/mL.