Biotin Monoclonal Antibody, IgG2a, Clone: [4C7.D5], Mouse

Produktübersicht

• Artikelname: Biotin Monoclonal Antibody, IgG2a, Clone: [4C7.D5], Mouse
• Artikelnummer: ROC-200-301-098
• Hersteller Artikelnummer: 200-301-098
• Alternativnummer: ROC-200-301-098
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Immunogen: Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).
• Konjugation: Unconjugated
• Alternative Synonym: mouse anti-Biotin Monoclonal Antibody, Biotin Antibody, Vitamin H, Coenzyme R

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
• Klon-Bezeichnung: [4C7.D5]
• Isotyp: IgG2a
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000, Flow Cytometry: 1-2 µg/mL, IHC: 1:1,000 - 1:5,000, IF Microscopy: 2 ug/mL, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Biotin antibody has been tested in ELISA, western blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specifi

Bilder

Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Gain: 800. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF(TM)488A anti-biotin secondary antibody at 2ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.

Western Blot of Mouse Anti-Biotin Monoclonal Antibody. Lane 1: Opal PreStained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Bovine Albumin (BSA) (p/n 001-0133) [20ng]. Lane 3: Bovine Albumin-Biotin conjugated (p/n 001-0633) [20ng). Primary Antibody: Anti-Biotin at 1.0µg/mL overnight at 2-8C. Secondary Antibody: Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated (p/n 610-403-C46) at 1:40,000 for 30mins at RT. Blocking Buffer: BlockOut Buffer (p/n MB-073) for 1hr at RT. Predicted MW: 75kDa. Exposure: 2sec.

Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF(TM)488A anti-biotin secondary antibody at 2ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.

Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Gain: 800. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF(TM)488A anti-biotin secondary antibody at 1ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.