6X His Tag Antibody HRP Conjugated, IgG1, Clone: [33D10.D2.G8], Mouse, Monoclonal

Produktübersicht

• Artikelname: 6X His Tag Antibody HRP Conjugated, IgG1, Clone: [33D10.D2.G8], Mouse, Monoclonal
• Artikelnummer: ROC-200-303-382
• Hersteller Artikelnummer: 200-303-382
• Alternativnummer: ROC-200-303-382
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: This 6X his antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
• Konjugation: HRP
• Alternative Synonym: mouse anti-6X His Tag HRP conjugated Antibody, peroxidase conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [33D10.D2.G8]
• Isotyp: IgG1
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or

Bilder

ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: 6X HIS-tagged conjugated BSA at the N or C terminus of the 6XHis. Coating amount: 0.15ug per well. Primary antibody (direct detection): HRP conjugated 6xHIS Tag antibody diluted from stock concentration at 100ug/mL. Substrate: TMB (p/n TMBE-1000).

ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-1000).

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.