Streptavidin Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Streptavidin Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Artikelnummer: ROC-200-403-095
• Hersteller Artikelnummer: 200-403-095
• Alternativnummer: ROC-200-403-095
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Bacteria
• Immunogen: Streptavidin [Streptomyces avidinii]
• Konjugation: HRP
• Alternative Synonym: rabbit anti-Streptavidin Antibody HRP Conjugation, Peroxidase conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin HRP Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000-1:10,000
• Anwendungsbeschreibung: Anti-Streptavidin Peroxidase antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at

Bilder

Western Blot Results of Rabbit Anti-Streptavidin Antibody Peroxidase Conjugated. Lane 1: Streptavidin Reduced [0.05µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Streptavidin Non-Reduced [0.05µg]. Primary Antibody: Anti-Streptavidin Peroxidase at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase (p/n 611-103-122) at 1:70,000 for 30mins at RT. Blocking Buffer: BlockOut Universal Blocking Buffer (p/n MB-073) for 1 hour at RT. Expect: Reduced- monomer ~17kDa, Non-Reduced- tetramer ~45kDa. Exposure: 5sec.