Beta Galactosidase Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Beta Galactosidase Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-200-4136-0100
• Hersteller Artikelnummer: 200-4136-0100
• Alternativnummer: ROC-200-4136-0100
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: Full length native Beta Galactosidase isolated from E.coli
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal antibody, ß-Gal, Anti-ß-Gal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 414878
• UniProt: P00722
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, IHC: 1:1,500, IF Microscopy: User Optimized, WB: 1:5,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Beta-Gal Antibody has been tested by ELISA and western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes bo

Bilder

Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: Beta-Galactosidase Reduced [0.1µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Beta-Galactosidase Non-Reduced [0.1µg]. Primary Antibody: Anti-Beta-Galactosidase at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) 1:70,000 for 30min at RT. Expected MW: ~117kDa.

Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: partially purified preparation b-Galactosidase [1.0µg]. Lane 2: Molecular Weight Marker. Primary Antibody: Anti-Beta-Galactosidase at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG IRDye800 (p/n 611-132-122) 1:10,000 for 45min at RT. Observed MW: ~117kDa.

Western blotting using Rocklands Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). The membrane was probed with fluorescein conjugated anti-b-Galactosidase (p/n 200-4236) diluted to 1:10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results