GST Antibody, Goat, Polyclonal

Produktübersicht

• Artikelname: GST Antibody, Goat, Polyclonal
• Artikelnummer: ROC-600-101-200
• Hersteller Artikelnummer: 600-101-200
• Alternativnummer: ROC-600-101-200
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
• Konjugation: Unconjugated
• Alternative Synonym: goat anti-GST antibody, Glutathione-S-Transferase

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.20 mg/mL by UV absorbance at 280 nm
• NCBI: 08515
• UniProt: P08515
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:50,000, IHC: 1:1,000 - 1:10,000, IP: User Optimized, WB: 1:1,000 - 1:10,000
• Anwendungsbeschreibung: Anti-GST has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This antibody has also been reported to be suitab

Bilder

ELISA results of purified Goat Anti-GST Antibody tested against GST. Each well was coated in 1.0 µg of antigen. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer (p/n MB-060-1000), Substrate (p/n TMB-8000), and Donkey Anti-Goat IgG Antibody HRP (p/n 605-703-125) at 1:15,000.

Western Blot of Goat Anti-GST Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: rec GST [0.1µg]/ HeLa Whole Cell Lysate [10µg]. Lane 3: rec GST [0.05µg]/ HeLa Whole Cell Lysate [10µg]. Lane 4: rec GST [0.02µg]/ HeLa Whole Cell Lysate [10µg]. Lane 3: HeLa Whole Cell Lysate (p/n W09-000-364) [10µg]. Primary Antibody: Goat anti-GST antibody (p/n 600-101-200) at 1.0µg/mL for overnight at 2-8C. Secondary Antibody: Rabbit Anti-Goat IgG (H&L) Antibody Peroxidase Conjugated (p/n 605-4302) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) at RT for 30 min. Predicted/Observed size: ~26kDa.

Immunoprecipitation of anti-GST antibody. Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 µg per lane. Primary antibody: anti-GST 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.

Western Blot of Goat anti-GST antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: CHO/K1 Lysate (p/n W07-000-357). Lane 4: MDA-MB-231 (p/n W09-001-GK6). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: Jurkat Lysate (p/n W09-001-370). Lane 7: NIH/3T3 Lysate (p/n W10-000-358). Lane 8: E-coli HCP Control (p/n 000-001-J08). Lane 9: FLAG Positive Control Lysate (p/n W00-001-383). Lane 10: Red Fluorescent Protein (p/n 000-001-379). Lane 11: Green Fluorescent Protein (p/n 000-001-215). Lane 12: Glutathione-S-Transferase Protein (p/n 000-001-200). Lane 13: Maltose Binding Protein (p/n 000-001-385). Load: 10 µg of lysate or 50ng of purified protein per lane. Primary antibody: GST antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody (p/n 605-4302) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 26 kDa for GST.