Anti-GST Antibody, Biotin Conjugated has been tested by ELISA and is suitable for immunoblotting, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Opti
ELISA results of purified Goat anti-GST Antibody Biotin Conjugated tested against purified GST. Each well was coated in duplicate with 1.0 µg of GST (p/n 000-001-200). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate p/n TMBE-1000
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