Anti-HMGB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid peptide near the internal region of human HMGB1.
Konjugation:
Unconjugated
Alternative Synonym:
HMGB1 Antibody, HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1
Anti-HMGB1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 25 kDa in Western Blots of specifi
Immunofluorescence of HMGB1. Cell: HeLa cells. Fixation: PFA-fixed. Primary Antibodies: HMGB1 at 20 µg/mL followed by goat anti-rabbit IgG antibody at 1:1000 dilution (red) and Hoechst staining (blue), alpha tubulin stained with anti-alpha tubulin antibody following by goat anti-mouse IgG antibody (green). Images were captured with confocal microscopy.
Immunohistochemistry of HMGB1. Tissue: Human Kidney Tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-HMGB1 antibody at 2 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Western Blot of HMGB1. Load: 15µg of rat tissue lysates. Primary Antibody: HMGB1 at 1µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of HMGB1. Tissue: Mouse Kidney Tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-HMGB1 antibody at 2 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Western Blot of HMGB1. Load: 15µg of mouse tissue lysates. Primary Antibody: HMGB1 at 1µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of HMGB1. Tissue: Rat Kidney Tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-HMGB1 antibody at 2 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Western Blot of HMGB1. Load: 10µg of HeLa WT or HMGB1 KO cell lysates. Primary Antibody: HMGB1 at 0.5µg/mL and beta-actin at 1µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of HMGB1. Tissue: Human Stomach Tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-HMGB1 antibody at 1 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Western Blot of HMGB1. Load: 15µg of human, mouse, or rat cell line lysates. Primary Antibody: HMGB1 at 1µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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