Anti-Rkhd4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from near the C-terminus of human Rkhd4. The immunogen is located within amino acids 420-470 of RKHD4.
Konjugation:
Unconjugated
Alternative Synonym:
Rkhd4 Antibody, RKHD4, MEX-3A, RKHD4, RNA-binding protein MEX3A, RING finger and KH domain-containing protein 4
Anti-RKHD4 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 54 kDa in Western Blots of specifi
Immunofluorescence Microscopy of Rkhd4 antibody. Tissue: Human brain tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Rkhd4 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Rkhd4 as a red fluorescent signal.
Immunohistochemistry of Rkhd4 antibody. Tissue: Human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Rkhd4 antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Rkhd4 is nuclear and occasionally cytoplasmic. Staining: Rkhd4 as a precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of Rkhd4 antibody. Lane A: SK-N-SH cell lysate at 1 µg/mL in the absence of blocking peptide. Lane B: SK-N-SH cell lysate at 1 µg/mL in the presence of blocking peptide. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 54.1 kDa, ~60 kDa for Rkhd4.
Western blot Validation of RKHD4. Load: 15 µg of human Daudi, Mouse spleen, or Rat Thymus lysate. Primary: antibody: RKHD4 at 2 µg/mL for 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of RKHD4. Tissue: Rat Testis. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-RKHD4 antibody at 1 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Immunohistochemistry Validation of RKHD4. Tissue: Mouse lung tissue. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. mouse lung tissue using Primary Antibody: anti-RKHD4 antibody at 1 µg/ml overnight at 4C. Antigen retrieval: heat mediation with a citrate buffer (pH6). Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Immunohistochemistry Validation of RKHD4. Tissue: Human Testis. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-RKHD4 antibody at 1 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
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