Beclin-1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to an internal region of human Beclin-1. The immunogen is located between 40-90 amino acids of Beclin-1.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
IHC: 2-5 µg/mL, IF Microscopy: 10-20 µg/mL, WB: 1-2 µg/mL
Anwendungsbeschreibung:
Anti-Beclin-1 Antibody is tested for use in ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence. Expect a band approximately ~51.8 kDa in western blot using positive control HEK293 whole cell lysates, mouse brain tissue for IHC and IF, or o
Immunofluorescence of Beclin-1. Cell: HeLa cells. Fixation: methanol-fixed. Primary Antibody: anti-Beclin-1 at 10 µg/mL. Secondary: goat anti-rabbit IgG secondary antibody at 1:1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Immunohistochemistry of Beclin-1. Tissue: Rat Liver Tissue. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-Beclin-1 antibody at 2 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Immunofluorescence of Beclin-1. Tissue: Mouse Brain Tissue. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: Anti-Beclin-1 at 20µg/mL.Secondary: goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI antibody (blue).
Immunohistochemistry of Beclin-1.Tissue: human kidney tissue. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-Beclin-1 antibody at 2 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Western Blot of Beclin-1.Load: 15 µg of lysate. Lane 1: mouse heart, Lane 2: mouse brain, Lane 3: mouse colon. Primary antibody: Anti-Beclin-1 at 1µg/mL for 1h at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of Beclin-1.Tissue: Mouse Brain Tissue. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-Beclin-1 antibody at 5 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Western Blot of Beclin-1. Load: 15 µg of lysate. Lane 1: rat liver, Lane 2: rat heart, Lane 3: rat lung, Lane 4: rat brain, Lane 5: rat ovary, Lane 6: rat colon. Primary antibody: anti-Beclin-1 at 1µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of Beclin-1. Tissue: Rat Brain Tissue. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: mediation with a citrate buffer (pH6). Primary Antibody: anti-Beclin-1 antibody (3613) at 2 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
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