VSV-G Epitope Tag Antibody Biotin Conjugated, Rabbit, Polyclonal

Artikelnummer: ROC-600-406-386
Artikelname: VSV-G Epitope Tag Antibody Biotin Conjugated, Rabbit, Polyclonal
Artikelnummer: ROC-600-406-386
Hersteller Artikelnummer: 600-406-386
Alternativnummer: ROC-600-406-386
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G).
Konjugation: Biotin
Alternative Synonym: Rabbit Anti-VSV-G Epitope Tag Biotin Conjugated Antibody, Rabbit Anti VSV-G Epitope Tag Biotin Conjugated Antibody, Rabbit Anti-VSV-G Tag Antibody Biotin Conjugation
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: This affinity purified antibody has been tested for use in ELISA and western blot and is suitable by immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluorescence assays, w
ELISA results of purified Rabbit anti-VSV-G Antibody Biotin Conjugated tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated (p/n S000-03) and TMB substrate p/n TMBE-1000.