Rabbit IgG anti-Goat IgG (H+L)-ATTO 488, MinX Ms,Rb, ATTO-488, Polyclonal

Produktübersicht

• Artikelname: Rabbit IgG anti-Goat IgG (H+L)-ATTO 488, MinX Ms,Rb, ATTO-488, Polyclonal
• Artikelnummer: ROC-605-452-013
• Hersteller Artikelnummer: 605-452-013
• Alternativnummer: ROC-605-452-013
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FLISA,IF,WB
• Spezies Reaktivität: Goat
• Immunogen: Goat IgG whole molecule
• Konjugation: ATTO 488
• Alternative Synonym: rabbit anti-Goat IgG ATTO 488 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO488 Conjugation
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Mouse,Rabbit

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Goat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
• Anwendungsbeschreibung: GOAT IgG (H&L) conjugated to ATTO 488 has been tested by western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for mu

Bilder

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

Western Blot of Rabbit anti-Goat IgG Atto488 Conjugated Antibody. Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Atto488 rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Goat IgG. Other band(s): None.