Produktbeschreibung
Phosphoglucomutase (PGM) interconverts the 1- and 6-phosphate isomers of alpha-D-glucose. Although it is a reversible reaction, the formation of glucose-6-phosphate is markedly favored. PGM will not convert beta-D-glucose-1-phosphate or beta-D-glucose-6-phosphate Protein: As Reported mg protein/mg solid Activity: As Reported Specific Activity: 100U/mg protein Unit Definition: One unit will catalyze the conversion of 1umole of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate/min at pH 7.4 at 30C. Solubility (1mg/ml in saline): Colorless to faint yellow, clear to slightly hazy Contaminants: LDH: 0.1% PK: 0.05% Phosphoglucose Isomerase: 1% Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with saline. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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