O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1-X-S-X-P-C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C-terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T-acute lymphoblastic leukemias (3). Recently, POGLUT1 has been demonstrated in a phosphatae- coupled glycosyltransferase assay to have hydrolase activity on UDP-Glc (4). Source: Recombinant corresponding to aa1-388 from human POGLUT, fused with 6-His tag at C-terminal, expressed in CHO cells. Molecular Weight: ~44kD Endotoxin Level: 1EU/1ug (LAL) Biological Activity: Recombinant corresponding to aa1-388 from human POGLUT CHO cell line, with a C-terminal 6-His tag expressed in CHO cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
