Description
Avidin DCS, Texas Red® labeled, is a cell sorter grade of Texas Red® Avidin D. Through the development of a new method of labeling we are able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Texas Red® Avidin DCS have a high F/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, we employ a purification step to reduce nonspecific binding which can hamper resolution in cell sorting and other applications.
Specifications
| Unit Size |
1 mg |
| Applications |
Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage |
For diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended range of concentration for staining of cells or tissues is 5-20 µg/ml. |
| Solution |
10 mM HEPES, 0.5 M NaCl, pH 7.5, 0.08% sodium azide, 10 mg/ml beta cyclodextrin |
| Maximum Excitation |
595-604 nm |
| Maximum Emission |
606-615 nm |
| Recommended Storage |
2-8 °C |
| Concentration |
2.0 mg active conjugate/ml |
| Conjugate |
Texas Red |
| Color of Fluorescence |
Red |
| Format |
Concentrate |