Description
Biotinylated Goat Anti-Rat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For mouse tissue applications, please see anti-rat, mouse adsorbed, secondary antibodies.)
- Recognizes both heavy and light chains (H+L)
- Supplied in solution
Specifications
| Unit Size |
1.5 mg |
| Applications |
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration |
1.5 mg active conjugate/ml |
| Recommended Storage |
2-8 °C; Store frozen for long term storage |
| Solution |
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. |
| Recommended Usage |
The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. NOTE: This product is raised in goat and is not the same as that contained in the VECTASTAIN ABC Kits for the detection of rat IgG which is raised in rabbit. |
| Target Species |
Rat |
| Conjugate |
Biotinylated |
| Host Species |
Goat |
| Format |
Concentrate |