Description
The biotinylated horse anti-goat IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
- Suitable for use with goat, sheep, and bovine IgG primary antibodies
- Recognizes both heavy and light chains (H+L)
- Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
- Supplied in solution
Specifications
| Unit Size |
1.5 mg |
| Applications |
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration |
1.5mg active conjugate/ml |
| Recommended Storage |
2-8 °C; Store frozen for long term storage |
| Solution |
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. |
| Recommended Usage |
The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Important Note:The use of bovine products such as casein, serum, albuminor non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present.If this antibody is to be used in these applications, blocking with Animal-Free Blocker™ solution (Cat. No. SP-5030) is recommended. NOTE: This product is raised in horse and is not the same as that contained in the VECTASTAIN ABC Kits for the detection of goat IgG which is raised in rabbit. |
| Target Species |
Goat |
| Conjugate |
Biotinylated |
| Host Species |
Horse |
| Format |
Concentrate |
