Calibration and Performance Tracking of Flow Cytometer

SpheroTechnical Notes #8

The SPHERO™ Calibration Particles are versatile, stable, economical and convenient to use. These particles contain a mixture of fluorochrome which are spectrally similar to many of the fluorochromes used in flow cytometry. As a result, they are used for routine alignment, day-to-day performance verification and long term performance tracking of several channels of flow cytometers in one run. These particles are very stable since the fluorochromes are entrapped inside the particles instead of being located on the surface. They are packaged in a convenient dropper bottle to facilitate the dispensing and storage. The diluted particles can be stored for later use if desired to reduce costs. These products and their uses are described briefly as follows:


Rainbow Calibration Particles (RCPs):
The RCPs are designed for the routine calibration of most available channels in any flow cytometer. For example, these particles are used to verify the instrument set up and to check the linearity and sensitivity of the instrument. If factory recommended procedures are used for instrument set up, we recommend that the RCPs are included into QC programs to track the long term and day-to-day performance.

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