Monoclonal Antibody to GAPDH (Clone: ABM22C5), Clone: [ABM22C5], Mouse
Artikelnummer:
ABI-10-10011
Hersteller Artikelnummer:
10-10011
Alternativnummer:
ABI-10-10011-100UG
Hersteller:
Abeomics
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC, WB
Spezies Reaktivität:
Human, Mouse
Immunogen:
A partial length recombinant GAPDH protein (amino acids 120-320) was used as the immunogen for this antibody.
Alternative Synonym:
GAPDH||GAPD||CDABP0047||OK/SW-cl.12
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.
Western blot analysis: 0.5-2 µg/ml, Immunohistochemical analysis: 5 µg/ml
Figure-1: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 0.5 µg/ml on (1) Panc-1, (2) EL-4, (3) Jurkat and (4) HepG2 lysates.
Figure-3: Immunohistochemical analysis of GAPDH in human Small intestine tissue using GAPDH antibody (Clone: ABM22C5) at 10 µg/ml.
Figure-2 : Immunohistochemical analysis of GAPDH in human Liver tissue using GAPDH antibody (Clone: ABM22C5) at 3 µg/ml.
Figure-4: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 4 µg/ml on (1) CHO-K1 and (2) CHO/PD1 transfected Lysates.
Figure-5: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 4 µg/ml on Raw cell Lysate.
Figure-6: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) h Lung, (2) h Testis, (3) h Liver and (4) h Ovary lysates.
Figure-7: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) h Brain, (2) m Brain, (3) h Pancrease, (4) h Kidney and (5) m Skeletal Muscle lysates.
Figure-8: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) PC3, (2) A549, (3)THP1, (4) Ramos, (5) HCT-116, (6) Raji and (7) MOLT-4 lysates.
Figure-9: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) U937, (2) Saos2, (3) A431, (4) K562, (5) U87, (6) MCF-7, (7) Snu1 and (8) Jurkat lysates.
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