NANOG Monoclonal Antibody, IgG1, Clone: [8A1D11], Unconjugated, Mouse

Produktübersicht

• Artikelname: NANOG Monoclonal Antibody, IgG1, Clone: [8A1D11], Unconjugated, Mouse
• Artikelnummer: CSB-MA888008A0M
• Hersteller Artikelnummer: CSB-MA888008A0m
• Alternativnummer: CSB-MA888008A0M-100UL, CSB-MA888008A0M-50UL
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, ICC, IF, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Konjugation: Unconjugated
• Alternative Synonym: Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [8A1D11]
• Isotyp: IgG1
• UniProt: Q9H9S0
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: NANOG

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200

Bilder

Overlay histogram showing Hela cells stained with CSB-MA888008A0m (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Overlay histogram showing MCF-7 cells stained with CSB-MA888008A0m (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Overlay histogram showing Ntera-2 cells stained with CSB-MA888008A0m (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunocytochemistry analysis of CSB-MA888008A0m diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry analysis of CSB-MA888008A0m diluted at 1:100 and staining in Ntera-2 cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-MA888008A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of Ntera-2 cells with CSB-MA888008A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Western Blot
Positive WB detected in: Rat brain tissue, Mouse brain tissue
All lanes: NANOG antibody at 1:500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 33 kDa
Observed band size: 46, 42 kDa

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Ntera-2 whole cell lysate, A549 whole cell lysate
All lanes: NANOG antibody at 1:500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 33 kDa
Observed band size: 46, 40 kDa