Discovery Research

Effective transfection solutions from Mirus Bio

Mirus Bio is the leading manufacturer of transfection reagents for chemical transfection, electroporation, and viral transduction, and offers a range of effective transfection solutions and tools for a comprehensive set of cell lines. These solutions are intentionally designed to be gentle on cells and maximize cell delivery while using a simplified, straightforward protocol.

 

Discovery Research Applications:

• CRISPR/Cas Genome Editing
• Stem Cell Transfection
• RNAi Gene Silencing
• mRNA and Viral RNA Transfection
• Stable Cell Line Generation
• Low Toxicity Transfection
• Co-transfection
• Nucleic Acid Labeling

 

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CRISPR/Cas Genome Editing

The bacterial CRISPR/Cas9 system has been adapted to serve as a versatile platform for RNA-directed genome editing in mammalian cells. The simplicity of using of a noncoding RNA guide to target DNA for site-specific cleavage provides a distinct advantage over alternative genome editing technologies such as ZFNs and TALENs. Using the CRISPR/Cas9 strategy, retargeting the nuclease complex only requires introduction of a new RNA sequence and there is no need to reengineer the specificity of DNA-binding proteins.

 

Discovery Research Products for CRISPR/Cas Genome Editing

TransIT-X2® Dynamic Delivery System

A novel, polymeric system for delivery of multiple nucleic acids to mammalian cells. Delivers CRISPR/Cas9 components in the following formats:

• DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
• Guide RNA – Deliver guide RNA oligonucleotides targeting your gene of interest
• RNP – Deliver Cas9/guide RNA ribonucleoprotein complexes

 

TransIT®-mRNA Transfection Kit

A high efficiency, low toxicity transfection reagent for mammalian cells. Delivers CRISPR/Cas9 components in the following formats:

• mRNA – Deliver messenger RNA expressing Cas9
• Guide RNA – Deliver guide RNA targeting your gene of interest

 

Ingenio® Electroporation Solution

A high efficiency electroporation solution compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®. Delivers CRISPR/Cas9 components in the following formats:

• DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
• mRNA – Deliver messenger RNA expressing Cas9
• Guide RNA – Deliver guide RNA oligonucleotides targeting your gene of interest
• RNP – Deliver Cas9/guide RNA ribonucleoprotein complexes

 

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