Discovery Research
Effective transfection solutions from Mirus Bio
Mirus Bio is the leading manufacturer of transfection reagents for chemical transfection, electroporation, and viral transduction, and offers a range of effective transfection solutions and tools for a comprehensive set of cell lines. These solutions are intentionally designed to be gentle on cells and maximize cell delivery while using a simplified, straightforward protocol.
Discovery Research Applications:
- CRISPR/Cas Genome Editing
- Stem Cell Transfection
- RNAi Gene Silencing
- mRNA and Viral RNA Transfection
- Stable Cell Line Generation
- Low Toxicity Transfection
- Co-transfection
- Electroporation
- Nucleic Acid Labeling
CRISPR/Cas Genome Editing
The bacterial CRISPR/Cas9 system has been adapted to serve as a versatile platform for RNA-directed genome editing in mammalian cells. The simplicity of using of a noncoding RNA guide to target DNA for site-specific cleavage provides a distinct advantage over alternative genome editing technologies such as ZFNs and TALENs. Using the CRISPR/Cas9 strategy, retargeting the nuclease complex only requires introduction of a new RNA sequence and there is no need to reengineer the specificity of DNA-binding proteins.
Discovery Research Products for CRISPR/Cas Genome Editing
TransIT-X2® Dynamic Delivery System
A novel, polymeric system for delivery of multiple nucleic acids to mammalian cells. Delivers CRISPR/Cas9 components in the following formats:
- DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
- Guide RNA – Deliver guide RNA oligonucleotides targeting your gene of interest
- RNP – Deliver Cas9/guide RNA ribonucleoprotein complexes
TransIT®-mRNA Transfection Kit
A high efficiency, low toxicity transfection reagent for mammalian cells. Delivers CRISPR/Cas9 components in the following formats:
- mRNA – Deliver messenger RNA expressing Cas9
- Guide RNA – Deliver guide RNA targeting your gene of interest
Ingenio® Electroporation Solution
A high efficiency electroporation solution compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®. Delivers CRISPR/Cas9 components in the following formats:
- DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
- mRNA – Deliver messenger RNA expressing Cas9
- Guide RNA – Deliver guide RNA oligonucleotides targeting your gene of interest
- RNP – Deliver Cas9/guide RNA ribonucleoprotein complexes
Stem Cell Transfection
Mirus Bio reagents enable high efficiency transfection of stem cells and other hard to transfect cell types used for stem cell research.
- Perform genome editing – TransIT-X2® Dynamic Delivery System
- Transfect DNA effectively – TransIT®-2020 Transfection Reagent or TransIT®-LT1 Transfection Reagent
- Perform repeated, low toxicity mRNA transfections – using TransIT®-mRNA Transfection Kit
- Electroporate efficiently and cost-effectively – with Ingenio® Electroporation Solution
RNAi Gene Silencing
The ability to silence genes plays an important role in molecular and cell biology and can be readily applied through transfection. Gene expression can be effectively reduced or eliminated by introducing small noncoding RNA molecules that inhibit RNA translation though a process termed RNA interference (RNAi).
RNA molecules that take part in naturally occurring RNAi pathways include:
- Small interfering RNAs (siRNA) – short (20-25 base pairs) double-stranded RNAs resulting from the cleavage of double-stranded RNAs (dsRNAs)
- MicroRNAs (miRNAs) – a separate class of short single-stranded RNAs (20-22 nucleotides) resulting from the processing of noncoding RNAs
Discovery Research Products for RNAi Gene Silencing
| Nucleic Acid to be Transfected | Transfection Reagent Recommendation |
|---|---|
| siRNA/miRNA |
Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT-TKO® Transfection Reagent Option 3: TransIT-siQUEST® Transfection Reagent |
| shRNA encoding Plasmid DNA libraries |
Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT®-2020 Transfection Reagent Option 3: TransIT®-LT1 Transfection Reagent |
| siRNA/miRNA and Plasmid DNA | TransIT-X2® Dynamic Delivery System |
mRNA and Viral RNA Transfection
While smaller RNA species such as siRNA/miRNA, etc. are routinely transfected by researchers investigating gene function, transfection of large RNA molecules such as mRNA transcripts and viral RNA replicons is an emerging trend. mRNA delivery for protein expression offers an inherent advantage as cytoplasmic delivery is sufficient to initiate translation of the transcript.
There have been tremendous improvement in in vitro transcription technology that facilitate transfection of RNA transcripts for prolonged and sustained protein expression. The TransIT®-mRNA Transfection Kit is formulated specifically for delivery of larger RNA species into mammalian cells.
Discovery Research Products for mRNA and Viral RNA Transfection
TransIT®-mRNA Transfection Kit
Each Kit is supplied with the TransIT®-mRNA Transfection Reagent and the mRNA Boost Reagent
- Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve RNA delivery in a large population of cells to ensure experimental success
- Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change and maintains cellular health
- Deliver Various Sizes of RNA – Ideal for specialized applications, such as virus production, protein expression and CRISPR genome editing
Stable Cell Line Generation
Most cell biology experiments utilize transient transfection protocols that afford peak gene expression between 24-96 hours post transfection. However, if sustained gene expression is required, generation of stable cell lines is a viable option. Furthermore, stable cell lines selected through limiting dilution/colony-picking provide a genetically homogenous and clonal population.
Discovery Research Products for Stable Cell Line Generation
TransIT-X2® Dynamic Delivery System
A high efficiency, low toxicity transfection reagent for large RNA delivery
- Efficiency –Exceptional broad spectrum transfection
- Versatility –Cutting edge delivery of plasmid DNA and small RNAs
- Technology – Novel, non-liposomal, polymeric delivery
TransIT®-LT1 Transfection Reagent
A low toxicity, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve expression in a large population of cells
TransIT®-2020 Transfection Reagent
A high performance, animal-free, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
- Animal Origin Free – High performance with maximum compatibility
Low Toxicity Transfection
Understanding the impact of transfection mediated toxicity is important for every researcher. Observed toxicity is largely dependent on the transfection reagent and cell type. Choosing a transfection reagent that balances high efficiency nucleic acid delivery and low cellular toxicity is imperative to achieving reliable experimental results. Providing researchers gene delivery expertise since 1995, Mirus TransIT® transfection reagents give researchers:
- High efficiency, low toxicity transfections
- Minimal off-target effects
- Reduction in experimental bias
Discovery Research Products for Low Toxicity Transfection
TransIT®-LT1 Transfection Reagent
A low toxicity, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve expression in a large population of cells
TransIT®-2020 Transfection Reagent
A high performance, animal-free, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
- Animal Origin Free – High performance with maximum compatibility
Co-transfection
Co-transfection or co-delivery of multiple nucleic acids – either similar or different in nature is a technique routinely employed by cell biologists to address increasingly complex experimental design and applications. Common examples of nucleic acid co-transfection are as follows:
- Co-delivery of multiple plasmid DNAs for virus production
- Co-transfection of plasmid DNA and siRNA to investigate gene function
- Co-delivery of plasmid DNA and mRNA for gene insertion, etc.
Co-transfection of different nucleic acid types such as large plasmid DNA and smaller siRNA/miRNAs can be challenging primarily due to the size and charge difference between these nucleic acid molecules. The charge of the nucleic acid molecule impacts the charge ratio of the transfection complexes and their formation and uptake by the mammalian cell membrane. Due to this reason, transfection reagents that can deliver smaller nucleic acids such as siRNA/miRNA to the cytoplasm efficiently are not always successful in plasmid DNA delivery to the nucleus. Unique polymeric formulations such as the TransIT-X2® Dynamic Delivery System are very effective nucleic acid condensing agents.
Discovery Research Products for Co-transfection
| Nucleic Acid(s) to be Co-transfected | Transfection Reagent |
|---|---|
| Multiple plasmid DNAs | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT®-2020 Transfection Reagent Option 3: TransIT®-LT1 Transfection Reagent |
| Multiple siRNAs | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT-TKO® Transfection Reagent Option 3: TransIT-siQUEST® Transfection Reagent |
| Plasmid DNA and siRNA | TransIT-X2® Dynamic Delivery System |
| Multiple mRNAs | TransIT®-mRNA Transfection Kit |
| Plasmid DNA and mRNA | TransIT-X2® Dynamic Delivery System and TransIT®-mRNA Transfection Kit |
| Multiple oligonucleotides | TransIT®-Oligo Transfection Reagent |
| Plasmid DNA and oligonucleotides | TransIT-X2® Dynamic Delivery System and TransIT®-Oligo Transfection Reagent |
Electroporation
Mirus Bio offers a full range of electroporation offerings, including; Ingenio® Electroporation Solution, electroporation cuvettes, and the new Ingenio® EZporator® Electroporation System. These products are designed to high efficiency delivery to hard-to-transfect cells without the cost or complication of other systems.
- High Efficiency Electroporation of Hard to Transfect Cells – Including primary cells and stem cells
- Compatible with Most Conventional Electroporation Devices – Including the Ingenio® EZporator®, Lonza-Amaxa®, Bio-Rad® and Harvard BTX®
- Cost Savings Using the Ingenio® Electroporation Kits – On your existing Amaxa® Nucleofector® without sacrificing performance
- Long Shelf Life – One year shelf life allows maximum usage for your experiments
- Buy Only What You Need – Available as solution alone or as part of a kit with cuvettes and cell droppers
Discovery Research Products for Electroporation
Ingenio® EZporator Electroporation System & Solution
The Ingenio® EZporator® Electroporation System is an easy-to-use electroporation system for mammalian cells.
- Easily optimize electroporation parameters for each cell type
- Every system comes with a complimentary Ingenio® Electroporation Kit
Nucleic Acid Labeling
With Label IT® fluorophores and labels, you can quickly and easily label any purified single or double-stranded nucleic acid that’s at least 5 nucleotides long.
Simply choose the right Label IT® reagent, add it to the nucleic acid, incubate for one hour*, and use the labeled nucleic acid in your choice of applications. The structure and functionality of your nucleic acid will remain intact, and the process can be used with even the most sensitive cell lines including hematopoietic stem and progenitor cells, iPSCs, and human T cells.
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