Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12), Clone: [ABM1C12], Mouse

Product Overview

• Article Name: Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12), Clone: [ABM1C12], Mouse
• Biozol Catalog Number: ABI-10-1003
• Supplier Catalog Number: 10-1003
• Alternative Catalog Number: ABI-10-1003-100UG
• Manufacturer: Abeomics
• Host: Mouse
• Category: Antikörper
• Application: FACS, IHC, WB
• Species Reactivity: Human
• Immunogen: Full length recombinant Caspase-3 protein was used as the immunogen for this antibody.
• Alternative Names: Casp3, Cpp32

Product Description

Caspases are a member of the cysteine-aspartic acid protease family. Caspase-3 (31 kDa) is an executionary caspase which directly cleaves and activates poly(ADP-ribose) polymerase (PARP), sterol regulatory element binding proteins (SREBPs) or it can also interact with other caspases like caspase-6, -7 and -9. Increased levels of caspase-3 are involved in Huntington Disease-associated cell death. Caspase-3 is the principal caspase in mediating the cleavage of amyloid-beta 4A precursor protein (APP), which is related with neuronal death in Alzheimers disease. Like other caspases, caspase-3 is also synthesized as a zymogen procaspase which is activated by specific proteolytic cleavage. High levels of caspase-3 are observed in lung, spleen, heart, liver and kidney, moderate levels in brain and skeletal muscle, and low in testis.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ABM1C12]
• NCBI: 836
• UniProt: P42574
• Purity: Protein G Chromatography
• Form: Purified
• Target: Caspase-3 (Pro and Active)
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Dilute: Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5-10 µg/ml, FACS analysis: 0.5-1 µg/10 6 cells

Images

Fig-6 :Immunohistochemical analysis of Caspase-3 in human Small Intestine tissue using Caspase-3 antibody (Clone: ABM1C12) at 10 µg/ml.

Fig-7 :Immunohistochemical analysis of Caspase-3 in human Tonsil tissue using Caspase-3 antibody (Clone: ABM1C12) at 10 µg/ml.

Fig-8: Intracellular FLOW cytometric analysis of Caspase 3 (Clone : ABM1C12) in Jurkat cells using 0.5 µg/10 6 cells of antibody. Goat anti-mouse PE conjugate was used as secondary antibody. Green represents isotope control (ABEOMICS), red represents anti-caspase 3 antibody.

Fig-3 :Immunohistochemical analysis of Caspase-3 in adenocarcinoma of rectum using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.

Fig 1: Immunohistochemical analysis of Caspase-3 in human colon tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.

Fig.2: Western blot analysis of Caspase-3. Anti- Caspase-3 (Clone: ABM1C12) was used at 2 µg/ml on Ramos lysate.

Fig-4 :Immunohistochemical analysis of Caspase-3 in human spleen tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.

Fig-5 :Immunohistochemical analysis of Caspase-3 in squamous cell carcinoma of lungs using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.