Human Alpha-Synuclein (A53T) Pre-formed Fibrils Protein, Tag Free, Unconjugated, E. coli
Biozol Catalog Number:
ABS-ALN-H5114-500UG
Supplier Catalog Number:
ALN-H5114-500ug
Alternative Catalog Number:
ABS-ALN-H5114-500UG
Manufacturer:
AcroBiosystems
Host:
E. coli
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Unconjugated
Alphasynuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membranebound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string proteinalpha/DNAJC5. Abnormalities in alphasynuclein are implicated in the pathogenesis of Parkinsons disease (PD). Alphasynuclein is present in Lewybodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration. - Proteine/Peptide
1. Sonication Conditions, Dissolution, Aliquoting, Storage, and Notes for PFF Thawing: Thaw PFFs rapidly in a 37 C water bath, or allow to thaw at room temperature. Aliquoting: Since PFFs are supplied as a suspension, pipette up and down thoroughly before aliquoting to ensure homogeneity. Storage: Store at -80 C at all times, avoid storage at 4 C or -20 C, which can induce fibril depolymerization. * alpha-Syn fibrils cold-denatured to monomers at 0-20 C and heat-denatured at 60-110 C. Sonication: The sonication protocols below are based on cell-based assay conditions. -Option 1 (Probe Sonicator): Use a probe sonicator (SCIENTZ) at 10% power (-95 W), applying 60 pulses of 0.5 s on/0.5 s off (recommended). -Option 2 (Ultrasonic Bath): Sonicate in an ultrasonic cleaner (40 kHz, 200-400 W) at 37 C for 1 hour, avoid performing the treatment at 4 C or 20 C. Note: The above sonication protocols are based on cell-based assays, other applications (e.g., in vivo injections) may require optimization-users should determine their optimal settings for their specific use case. 2. Recommendations for PFF Use in Animal Models Minimize Freeze-Thaw Cycles: For animal studies, it is recommend to use the PFF at once or aliquot before use to avoid repeated freeze-thaw cycles. PFF Maintenance During Injections: During stereotaxic injections, keep the sonicated PFFs in a 37 C water bath to prevent re-aggregation or sedimentation.
ALN-H5114-500ug
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