FGF2 Antibody - middle region
Catalog Number:
ASB-ARP42005_P050
- Images (9)
| Article Name: | FGF2 Antibody - middle region |
| Biozol Catalog Number: | ASB-ARP42005_P050 |
| Supplier Catalog Number: | ARP42005_P050 |
| Alternative Catalog Number: | ASB-ARP42005_P050-25UL,ASB-ARP42005_P050-100UL |
| Manufacturer: | Aviva |
| Host: | Rabbit |
| Category: | Proteine/Peptide |
| Application: | IHC, WB |
| Species Reactivity: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the middle region of human FGF2 |
| Alternative Names: | BFGF, FGFB, FGF-2, HBGF-2 |
| The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The peptide is present in isoforms of 31, 23, 21 and 17 kDa. The protein is processed to 16 kDa mature form, and the protein may be modified via methylation, phosphorylation and/or sumoylation. |
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Sample Type: Human A375 cellsPrimary Antibody Dilution: 1:100Secondary Antibody: Anti-rabbit-Alexa-546Secondary Antibody Dilution: 1:100Color/Signal Descriptions: Red: FGF2 Blue: NucleiGene Name: FGF2Submitted by: Igor Prudovsky, PhD, DSc, Principal Investigator, Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Dr. Scarborough, ME 4074 |
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WB Suggested Anti-FGF2 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Jurkat cell lysate |
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Host: Mouse Target Name: FGF2 Sample Tissue: Mouse Skeletal Muscle Antibody Dilution: 1ug/ml |
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WB Suggested Anti-FGF2 Antibody Titration: 2 ug/ml ELISA Titer: 1:312500 Positive Control: Hela cell lysate |
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Rabbit Anti-FGF2 Antibody Catalog Number: ARP42005_P050 Formalin Fixed Paraffin Embedded Tissue: Human Ovary Tissue Observed Staining: Nucleus Primary Antibody Concentration: 1:600 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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Host: Rabbit Target Name: FGF2 Sample Tissue: Human Lung Tumor Antibody Dilution: 1ug/ml |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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FGF2 antibody - middle region (ARP42005_P050) in Mouse Skeletal Muscle using Western Blot |
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