SLC15A4 Antibody - N-terminal region

Product Overview

• Article Name: SLC15A4 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP44108_P050
• Supplier Catalog Number: ARP44108_P050
• Alternative Catalog Number: ASB-ARP44108_P050-25UL,ASB-ARP44108_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SLC15A4
• Alternative Names: PHT1, PTR4, FP12591

Product Description

The function remains known.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 62 kDa
• NCBI: 121260
• UniProt: Q8N697
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Human Lung

WB Suggested Anti-SLC15A4 Antibody Titration: 0.25ug/ml
Positive Control: HepG2 cell lysate

Host: Rabbit
Target Name: SLC15A4
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: SLC15A4
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: SLC15A4
Sample Tissue: Human DLD1 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: SLC15A4
Sample Tissue: Human HCT116 Whole Cell
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 4 ug/mL of the antibody was used in this experiment. Recommended dilution for this antibody is 1-3 ug/mL. Protein is phosphorylated.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

Western Blot