GOLGB1 Antibody - N-terminal region

Product Overview

• Article Name: GOLGB1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP46464_P050
• Supplier Catalog Number: ARP46464_P050
• Alternative Catalog Number: ASB-ARP46464_P050-25UL,ASB-ARP46464_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GOLGB1
• Alternative Names: GCP, GCP372, GOLIM1

Product Description

GOLGB1 may participate in forming intercisternal cross-bridges of the Golgi complex.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 376 kDa
• NCBI: 2804
• UniProt: Q14789
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling Giantin with ARP46464_P050 at 5ug/ml.

WB Suggested Anti-GOLGB1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: 721_B cell lysateGOLGB1 is supported by BioGPS gene expression data to be expressed in 721_B

Host: Rabbit
Target Name: GOLGB1
Sample Tissue: Human PANC1 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: GOLGB1
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 4ug/ml

Host: Rabbit
Target Name: GOLGB1
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: GOLGB1
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: GOLGB1
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 3ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

GOLGB1 antibody - N-terminal region (ARP46464_P050) in Human 721_B using Western Blot