Bcl-2 Mouse Monoclonal Antibody, Clone: [8B5], Unconjugated

Product Overview

• Article Name: Bcl-2 Mouse Monoclonal Antibody, Clone: [8B5], Unconjugated
• Biozol Catalog Number: BYT-ORB499777
• Supplier Catalog Number: orb499777
• Alternative Catalog Number: BYT-ORB499777-50,BYT-ORB499777-100,BYT-ORB499777-200
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Bcl-2 (51-150/239aa)
• Conjugation: Unconjugated
• Alternative Names: Bcl-2, PPP1R50, BCL2_HUMAN, BCL2,

Product Description

Bcl-2 Mouse Monoclonal Antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [8B5]
• Molecular Weight: 26 kDa
• UniProt: P10415
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: BCL2

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bcl-2) Monoclonal Antibody, Unconjugated (orb499777) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (orb499777) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bcl-2) Monoclonal Antibody, Unconjugated (orb499777) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bcl-2) Monoclonal Antibody, Unconjugated (orb499777) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Jurkat (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-Bcl-2 (orb499777) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 26 kD.

Sample: LOVO (Human) Cell Lysate at 30 ug, Primary: Anti-Bcl-2 (orb499777) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 26 kD.

Sample: LOVO Cell (Human) Lysate at 40 ug, MCF-7 Cell (Human) Lysate at 40 ug, Primary: Anti-Bcl-2 (orb499777) at 1/2 000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 28 kD.

IHC-P analysis of human colon carcinoma using Bcl-2 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of rat kidney using Bcl-2 Monoclonal Antibody at 1:200 dilution.