ANXA11 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ANXA11 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA001838ESR2HU
• Supplier Catalog Number: CSB-PA001838ESR2HU
• Alternative Catalog Number: CSB-PA001838ESR2HU-100UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: 56 kDa autoantigen antibody, Annexin A11 antibody, Annexin XI antibody, Annexin-11 antibody, AnnexinA11 antibody, AnnexinXI antibody, ANX 11 antibody, ANX A11 antibody, ANX11 antibody, ANX11_HUMAN antibody, ANXA 11 antibody, ANXA11 antibody, Autoantigen 56 kD antibody, Calcyclin associated annexin 50 antibody, Calcyclin-associated annexin 50 antibody, CAP 50 antibody, CAP-50 antibody, CAP50 antibody, OTTHUMP00000059806 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P50995
• Buffer: Preservative: 0.02% sodium azide<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: ANXA11

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:3000, IHC:1:50-1:200, IF:1:20-1:100

Images

Immunohistochemist

Immunofluorescence staining of A549 cell with CSB-PA001838ESR2HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ImmunofluorescencestainingofA549cellwith5%goatserum,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasAlexaFluor488-congugatedAffiniPureGoatAnti-RabbitIgG(H+L).

Immunofluorescence staining of Hela cell with CSB-PA001838ESR2HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA001838ESR2HU diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA001838ESR2HU diluted at 1:50 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: Hela whole cell lysate(20µg), A431 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), A549 whole cell lysate(20µg), JK whole cell lysate(20µg), HepG2 whole cell lysate(20µg),MCF7 whole cell lysate(20µg),U251 whole cell lysate(20µg),
All lanes: ANXA11 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 54,51,56,60 kDa
Observed band size: 54,56 kDa
Exposure time: 20s