NQO2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: NQO2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA016040LA01HU
• Supplier Catalog Number: CSB-PA016040LA01HU
• Alternative Catalog Number: CSB-PA016040LA01HU-100UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: DHQV antibody, DIA6 antibody, EC 1.10.99.2 antibody, MGC94180 antibody, NAD(P)H dehydrogenase quinone 2 antibody, NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 antibody, NAD(P)H menadione oxidoreductase 2 dioxin inducible antibody, NMOR2 antibody, NQO 2 antibody, NQO2 antibody, NQO2_HUMAN antibody, NRH dehydrogenase [quinone] 2 antibody, NRH dehydrogenase antibody, NRH:quinone oxidoreductase 2 antibody, OTTHUMP00000015948 antibody, OTTHUMP00000015949 antibody, OTTHUMP00000015953 antibody, Ox 2 antibody, Ox2 antibody, QR2 antibody, Quinone antibody, Quinone reductase 2 antibody, Ribosyldihydronicotinamide dehydrogenase [quinone] antibody, Ribosyldihydronicotinamide dehydrogenase antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P16083
• Buffer: Preservative: 0.02% sodium azide<br />Constituents: PBS containing 50% glycerol pH 7.3
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: NQO2

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:4000, IHC:1:100-1:300, IF:1:20-1:100

Images

Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA016040LA01HU at dilution of 1:100

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, A549 whole cell lysate, MCF7 whole cell lysate, ntera2 whole cell lysate
All lanes:NQO2 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size:26 kDa
Observed band size:26 kDa

Immunofluorescence stainingof Hela cell with CSB-PA016040LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence stainingof U251 cell with CSB-PA016040LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA016040LA01HU diluted at 1:150 and staining in paraffin-embedded human breast tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA016040LA01HU diluted at 1:150 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody