Recombinant Human ADAR Protein (Full length, His & GST Tag)

Produktübersicht

• Artikelname: Recombinant Human ADAR Protein (Full length, His & GST Tag)
• Artikelnummer: SCM-A611-310GH
• Hersteller Artikelnummer: A611-310GH
• Alternativnummer: SCM-A611-310GH-10,SCM-A611-310GH-50,SCM-A611-310GH-500
• Hersteller: SignalChem
• Kategorie: Proteine/Peptide

Produktbeschreibung

Recombinant full-length human ADAR1 (p110) was expressed by baculovirus in Sf9 insect cells using an N-terminal His-GST tag.

Zusatzinformationen

Product Description

Recombinant full-length human ADAR1 (p110) was expressed by baculovirus in Sf9 insect cells using an N-terminal His-GST tag. The gene accession number is NM_001025107.3.

 

Scientific Background

Adenosine deaminases acting on RNA (ADARs) are enzymes that catalyze the chemical conversion of denosines to inosines in double-stranded RNA (dsRNA) substrates. ADAR1 (p110) is a ubiquitously expressed isoform of the ADAR1 enzyme localized primarily in the nucleus (1). It contains double-stranded RNA-binding domains and a catalytic deaminase domain, enabling it to edit RNA by converting adenosine to inosine in double-stranded RNA regions. This RNA editing regulates processes such as alternative splicing, transcript stability, and protein diversity. ADAR1 (p110) is essential for maintaining proper gene expression and neural function and plays a role in the cellular stress response (2). Dysfunctions in ADAR1 (p110) activity have been implicated in tumor suppressive properties (3).

References

1. Song, B. et al.: The role of RNA editing enzyme ADAR1 in human disease. WIRES RNA. 2022;13(1):e1665.

2. Shiromoto, S. M. et al.: Y. ADAR1 controls apoptosis of stressed cells by inhibiting Staufen1-mediated mRNA decay. Nature Structural and Molecular Biology. 2017; 24(6), 534–543.

3. Gumireddy, K. et al.: The mRNA-edited form of GABRA3 suppresses GABRA3-mediated Akt activation and breast cancer metastasis. Nature Communications. 2016; 7(1), 1–9.

 

Formulation

Recombinant protein stored 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.

 

Storage and Stability

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

 

 

Bilder

Recombinant human ADAR1 (p110) (Cat. No.: A611-310GH) showed dose-dependent adenosine deaminase activity towards double-stranded RNA in an ELISA format assay.

The purity of ADAR1 (p110) was determined to be >70% by densitometry. Calculated MW ~146 kDa Observed MW ~138 kDa