PYCARD Peptide

Catalog Number: PRS-45-129P
Article Name: PYCARD Peptide
Biozol Catalog Number: PRS-45-129P
Supplier Catalog Number: 45-129P
Alternative Catalog Number: PRS-45-129P-0.1
Manufacturer: ProSci
Category: Proteine/Peptide
Application: Blocking
Alternative Names: ASC, TMS, TMS1, CARD5, TMS-1, ASC, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, hASC
NCBI: 29108
UniProt: Q9ULZ3
Buffer: PYCARD peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
Form: Lyophilized
Application Notes: PYCARD Peptide is used for blocking the activity of PYCARD antibody (catalog number 45-129). The PYCARD antibody ships at 0.5 mg/mL and the PYCARD Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.