In unserem Journal Club stellen wir Ihnen regelmäßig aktuelle Publikationen mit relevanten Methoden und Produkten vor.
Polyglutamylation of tubulin's C-terminal tail controls pausing and motility of kinesin-3 family member KIF1A
Journal of Biological Chemistry, 2019
Recently, Lessard et al. investigated how polyglutamylation of tubulin’s C-terminal tails (CTTs) regulates processivity of the neuron-specific kinesin motor KIF1A. KIF1A transports neuronal cargo anterogradely along axonal microtubules (MTs) over long distances (~3 microns). Prior studies implicated
Different immune response of dendritic cells of newborns of allergic and healthy mothers to bacterial stimuli
Folia microbiologica, 2019
Tregs are in the center of research interest for many reasons resulting from their both physiological and pathological roles. Their main function is to suppress inadequate immune responses either to autoantigens or relatively innocuous environmental antigens such as pollen, dust, mites, antigens present in food or compounds of our microbiota. You can easily imagine a broad spectra of diseases related to impaired function of Tregs.
Fragmented mitochondria released from microglia trigger A1 astrocytic response and propagate inflammatory neurodegeneration
Nature Neuroscience 22, (2019)
Efforts to reduce the personal and societal burden of neurodegenerative disease (ND) hinge on the identification of the underlying molecular mechanism(s) that causes neuronal death. One common aspect of neuronal death in Alzheimer’s disease (AD), amyotrophic lateral sclerosis (ALS), and Huntington’s disease (HD) is the relationship to the buildup of neurotoxic proteins. Glial cells, normally neuroprotective, can be transformed into a neuroinflammatory state.
Structural determination of the large photosystem II–light-harvesting complex II supercomplex of Chlamydomonas reinhardtii using nonionic amphipol
The Journal of Biological Chemistry 294, 2019
Recently, the application of NAPol for the cryo-EM structure determination of membrane proteins was demonstrated and the Minagawa Lab published the structure of the large photosystem II-light harvesting complex II supercomplex of Chlamydomonas reinhardtii in August, 2019. A NAPol-based purification method was able to yield the largest photosynthetic supercomplex to the highest percentage of intact configuration reported to date!
Expansion and differentiation of hepatocyte-derived liver progenitor-like cells
Nature Cell Research, 2019
Stem cells are a better model of human disease and drug reactions than animal models. Fu et al. showed that human hepatocytes could be efficiently converted to progenitor-like cells by culturing in TEM, supplemented with small molecules that enable direct reprogramming. Some of those were contributed by our partner TargetMol.
CD8+ T cells induce cachexia during chronic viral infection
Nature Immunology, May 2019
Cachexia is a major cause of morbidity and mortality for various cancers, chronic inflammation, and infections. In this study, the authors used the chronic viral infection mice model to elucidate the inflammatory drivers of IAC. The free thyroxine levels of both infected and uninfected mice were measured by an ELISA kit from CUSABIO.
Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities
PLOS ONE, Oct 2017
The most common use of MHC tetramers is for detection of antigen-specific T cells. Ellen M. Leitman et al. describe the use of tetramers coupled to a Saporin to selectively kill antigen-specific T cells. These tetramers have the potential to be used in immunotherapy and vaccine development.
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