Application: FC (QC tested)

Reactivity: Human

Description

DryFlowEx PNH High-Sensitivity Assay Kit is intended for high sensitivity detection and enumeration of glycosyl-phosphatidyl-inositol (GPI)-deficient cells in human whole blood by flow cytometry.

Contents

The device DryFlowEx PNH High-Sensitivity Assay Kit, sufficient for examination of 25 patients, is provided with the following reagents:

• PNH High-Sensitivity Assay (25 pouches). Each pouch consists of 1 color-coded (Cyan strip) capped single-use tube PNH WBC 7-color (ED7750-1) and 1 color-coded (Red strip) capped single-use tube PNH RBC 3-color (ED7750-2), containing premixed combinations of fluorochrome-labeled reagents dried with the stabilizing ingredients as a layer at bottom of the test tubes (12 x 75 mm).
• Lysing Solution ED7750-3 (1 bottle) containing 15 ml of concentrated (10X) formaldehyde-based buffered solution.
• PNH Compensation Set ED7750-4 (1 pouch) containing 10 capped single-use tubes, each containing single fluorochrome-labeled reagent dried with the stabilizing ingredients as a layer at the bottom of the tube (12 x 75 mm).

Storage and handling: Store at 20-30°C. Protect from moisture.

 

The product is intended for In Vitro Diagnostic Use. In vivo diagnostic or therapeutic applications are strictly forbidden.

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Flow cytometry gating strategy for identification of target cell populations in normal human peripheral whole blood stained by PNH RBC 3-color.
Target cell populations:
CD59 dim CD235a+ Type II Erythrocytes
CD59- CD235a+ Type III Erythrocytes
CD59 dim CD235a+ CD71+ Type II Reticulocytes
CD59- CD235a+ CD71+ Type III Reticulocytes

 

Flow cytometry gating strategy for identification of target cell populations in normal human peripheral whole blood stained by PNH WBC 7-color.
Target cell populations:
CD14- CD157- CD64+ monocytes
CD14- Proaerolysin- CD64+ monocytes
CD157- Proaerolysin- CD64+ monocytes
CD24- CD157- CD15+ neutrophil granulocytes
CD24- Proaerolysin- CD15+ neutrophil granulocytes
CD157- Proaerolysin- CD15+ neutrophil granulocytes