1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purified by antigen-affinity chromatography.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4-8ºC. For long-term storage, aliquot and store at -20-8ºC or below. Avoid multiple freeze-thaw cycles.
WB: 1:1000-1:10000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications., IHC-Fr: 1:100-1:1000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other...
An ELISA plate is coated with 50 μL of a IL10 peptide at concentration ranged from 0.25 μg/mL to 8 μg/mL. The coated peptide is detected with anti IL10 antibody (GTX130513) at 0.5 μg/mL.
IL10 antibody detects IL10 protein by western blot analysis. Non-transfected (-) and IL10-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody (GTX130513) diluted at 1:5000.
IL10 antibody detects IL10 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse hippocampus.Green: IL10 stained by IL10 antibody (GTX130513) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
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