IL10 antibody

Artikelnummer: GTX-GTX130513
Artikelname: IL10 antibody
Artikelnummer: GTX-GTX130513
Hersteller Artikelnummer: GTX130513
Hersteller: Genetex
Wirt: Rabbit
Applikation: WB, IHC-Fr, ELISA
Spezies Reaktivität: Human, Mouse
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IL10. The exact sequence is proprietary.
Alternative Synonym: Interleukin 10,Csif,Gvhds,Il-10,Il10A,Tgif,Il10
Klonalität: Polyclonal
Konzentration: 2.16 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 21
NCBI: 3586
UniProt: P22301
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4-8ºC. For long-term storage, aliquot and store at -20-8ºC or below. Avoid multiple freeze-thaw cycles.
Anwendungsbeschreibung: WB: 1:1000-1:10000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications., IHC-Fr: 1:100-1:1000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other...
 An ELISA plate is coated with 50 μL of a IL10 peptide at concentration ranged from 0.25 μg/mL to 8 μg/mL. The coated peptide is detected with anti IL10 antibody (GTX130513) at 0.5 μg/mL.
 IL10 antibody detects IL10 protein by western blot analysis. Non-transfected (-) and IL10-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody (GTX130513) diluted at 1:5000.
 IL10 antibody detects IL10 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL10 stained by IL10 antibody (GTX130513) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).