IL10 antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX130513
Artikelname: IL10 antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX130513
Hersteller Artikelnummer: GTX130513
Alternativnummer: GTX130513-25,GTX130513-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB, IHC-Fr, ELISA
Spezies Reaktivität: Human, Mouse
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IL10. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: CSIF, cytokine synthesis inhibitory factor, GVHDS, IL-10, IL10A, interleukin-10, T-cell growth inhibitory factor, TGIF
Klonalität: Polyclonal
Konzentration: 2.16 mg/ml (Please refer to the vial label for the specific concentration.)
Isotyp: IgG
NCBI: 3586
UniProt: P22301
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: interleukin 10
Anwendungsbeschreibung: WB: 1:1000-1:10000. IHC-Fr: 1:100-1:1000. ELISA: 1:-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
 An ELISA plate is coated with 50 μL of a IL10 peptide at concentration ranged from 0.25 μg/mL to 8 μg/mL. The coated peptide is detected with anti IL10 antibody (GTX130513) at 0.5 μg/mL.
 IL10 antibody detects IL10 protein by western blot analysis. Non-transfected (-) and IL10-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody (GTX130513) diluted at 1:5000.
 IL10 antibody detects IL10 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL10 stained by IL10 antibody (GTX130513) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).