IL1 beta antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX74034
Artikelname: IL1 beta antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX74034
Hersteller Artikelnummer: GTX74034
Alternativnummer: GTX74034-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Functional Assay, IHC
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant murine IL1 beta
Konjugation: Unconjugated
Alternative Synonym: IL-1 beta, Il-1b, IL-1beta, interleukin-1 beta
Klonalität: Polyclonal
Konzentration: 2.07 mg/ml (Please refer to the vial label for the specific concentration.)
Isotyp: IgG
NCBI: 16176
UniProt: P10749
Puffer: PBS
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: interleukin 1 beta
Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. ELISA: 0.5-2µg/ml. Functional Assay: Assay dependent. IHC: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
 IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
 IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
 IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.
Red: NF-H, a neuron marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Untreated (–) and treated (+) THP-1 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
 Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX132080) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 An ELISA plate is coated with 50 μL of IL1 beta recombinant protein at concentration of 5 μg/mL. The coated protein is detected with anti-IL1 beta antibody (GTX74034) at concentration ranged from 0.01 to 0.15 μg/mL.
 Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.