Iba1 antibody

Artikelnummer: GTX-GTX100042
Artikelname: Iba1 antibody
Artikelnummer: GTX-GTX100042
Hersteller Artikelnummer: GTX100042
Alternativnummer: GTX100042-100, GTX100042-25
Hersteller: Genetex
Wirt: Rabbit
Applikation: WB, IF, ICC, IHC-P, IHC-Fr, FACS, IHC
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.
Konjugation: IgG
Alternative Synonym: Allograft Inflammatory Factor 1,Aif-1,Iba1,Irt-1,Irt1,Aif1

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

 

Application Reference

Hong Li et al. bioRxiv 2019; (Epub) C-terminal binding proteins 1 and 2 in traumatic brain injury-induced inflammation and their inhibition as an approach for anti-inflammatory treatment.

Application : IHC-Fr

Reactivity : Mouse

Olechnowicz SWZ et al. Sci Rep 2019; 9 (1) : 14189 Multiple myeloma increases nerve growth factor and other pain-related markers through interactions with the bone microenvironment.

Application : IHC-P

Reactivity : Mouse

Pejman S et al. Brain Res Bull 2019; Ac-SDKP ameliorates the progression of experimental autoimmune encephalomyelitis via inhibition of ER stress and oxidative stress in the hippocampus of C57BL/6 mice.

Application : IHC-P

Reactivity : Mouse

Bai KJ et al. Chem Biol Interact 2019; 311 : 108762 Microglial activation and inflammation caused by traffic-related particulate matter.

Application : IHC-P

Reactivity : Rat

Zhang T et al. FASEB J 2019; : fj201802614RRR Erianin alleviates diabetic retinopathy by reducing retinal inflammation initiated by microglia cells via inhibiting hyperglycemia-mediated ERK1/2-NF-庥B signaling pathway.

Application : WB, IHC

Reactivity : Rat

Klonalität: Polyclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 17
Isotyp: IgG
NCBI: 199
UniProt: P55008
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4-8ºC. For long-term storage, aliquot and store at -20-8ºC or below. Avoid multiple freeze-thaw cycles.
Anwendungsbeschreibung: WB: 1:500-1:10000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications., ICC/IF: 1:100-1:1000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other...
 Iba1 antibody detects Iba1 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:400. Blue: Hoechst 33342 staining.
 Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1(GTX100042) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
 Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Iba1 antibody (GTX100042) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
 Iba1 antibody detects Iba1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
15 % SDS-PAGE
Iba1 antibody (GTX100042) dilution: 1:1000
 Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.
 Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
 Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Iba1 antibody (GTX100042) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
 Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.
 Iba1 antibody (GTX100042) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (GTX100042) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
 Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.