GFAP antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX108711
Artikelname: GFAP antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX108711
Hersteller Artikelnummer: GTX108711
Alternativnummer: GTX108711-25,GTX108711-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB, ICC/IF, IHC-P, IHC-Fr, IHC
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: ALXDRD, glial fibrillary acidic protein
Klonalität: Polyclonal
Konzentration: 1.13 mg/ml (Please refer to the vial label for the specific concentration.)
Isotyp: IgG
NCBI: 2670
UniProt: P14136
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: glial fibrillary acidic protein
Anwendungsbeschreibung: WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IHC: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
 GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
 Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:50000.
 Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
 GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
 GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
 GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
 GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: GFAP antibody (GTX108711) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 GFAP antibody detects GFAP protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:300.
Blue: Hoechst 33342 staining.
 GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min