GFAP antibody

Artikelnummer: GTX-GTX108711
Artikelname: GFAP antibody
Artikelnummer: GTX-GTX108711
Hersteller Artikelnummer: GTX108711
Hersteller: Genetex
Wirt: Rabbit
Applikation: WB, IF, ICC, IHC-P, IHC-Fr, IHC
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.
Konjugation: IgG
Alternative Synonym: Glial Fibrillary Acidic Protein,Alxdrd,Gfap
Klonalität: Polyclonal
Konzentration: 1.41 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 50
Isotyp: IgG
NCBI: 2670
UniProt: P14136
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4-8ºC. For long-term storage, aliquot and store at -20-8ºC or below. Avoid multiple freeze-thaw cycles.
Anwendungsbeschreibung: WB: 1:5000-1:50000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications., ICC/IF: 1:100-1:1000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other...
 Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:50000.
 Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
 GFAP antibody detects GFAP protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
 GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
 GFAP antibody detects GFAP protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
 GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: GFAP antibody (GTX108711) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min