COX4 antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX114330
Artikelname: COX4 antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX114330
Hersteller Artikelnummer: GTX114330
Alternativnummer: GTX114330-25,GTX114330-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB, ICC/IF, IHC-P, IHC-Fr
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: COX IV-1, COX4, COX4-1, COXIV, COXIV-1, cytochrome c oxidase polypeptide IV, cytochrome c oxidase subunit 4 isoform 1, mitochondrial, cytochrome c oxidase subunit IV, Cytochrome c oxidase subunit IV isoform 1
Klonalität: Polyclonal
Konzentration: 0.42 mg/ml (Please refer to the vial label for the specific concentration.)
Isotyp: IgG
NCBI: 1327
UniProt: P13073
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: cytochrome c oxidase subunit 4I1
Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PMID: 29233981">  The IHC-P analysis of COX4 antibody was published by Fischer C and colleagues in the journal Nat Commun in 2017 ._x000D_PMID: 29233981
PMID: 29233981">  The IHC-P analysis of COX4 antibody was published by Fischer C and colleagues in the journal Nat Commun in 2017 ._x000D_PMID: 29233981
PMID: 29233981">  The IHC-P analysis of COX4 antibody was published by Fischer C and colleagues in the journal Nat Commun in 2017 ._x000D_PMID: 29233981
PMID: 29233981">  The IHC-P analysis of COX4 antibody was published by Fischer C and colleagues in the journal Nat Commun in 2017 ._x000D_PMID: 29233981
 COX4 antibody detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.
 COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.
 Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Sample (50 μg of whole cell lysate)
A: mouse muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Sample (50 μg of whole cell lysate)
A: rat muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.