COX4 antibody

Artikelnummer: GTX-GTX114330
Artikelname: COX4 antibody
Artikelnummer: GTX-GTX114330
Hersteller Artikelnummer: GTX114330
Hersteller: Genetex
Wirt: Rabbit
Applikation: WB, IF, ICC, IHC-P
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.
Konjugation: IgG
Alternative Synonym: Cytochrome C Oxidase Subunit 4I1,Cox Iv-1,Cox4,Cox4-1,Coxiv,Coxiv-1,Cox4I1
Klonalität: Polyclonal
Konzentration: 0.23 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 20
Isotyp: IgG
NCBI: 1327
UniProt: P13073
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4-8ºC. For long-term storage, aliquot and store at -20-8ºC or below. Avoid multiple freeze-thaw cycles.
Anwendungsbeschreibung: WB: 1:500-1:3000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications., ICC/IF: 1:100-1:1000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other...
 Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COX4 protein stained by COX4 antibody (GTX114330) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
 Immunohistochemical analysis of paraffin-embedded HS578T xenograft, using COX4(GTX114330) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
 Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4(GTX114330) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
 Sample (50 μg of whole cell lysate)
A: mouse muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Sample (50 μg of whole cell lysate)
A: rat muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.