SOD1 (mutant) antibody [MS785]

Artikelnummer: GTX-GTX57211
Artikelname: SOD1 (mutant) antibody [MS785]
Artikelnummer: GTX-GTX57211
Hersteller Artikelnummer: GTX57211
Hersteller: Genetex
Wirt: Rat
Kategorie: Antikörper
Applikation: WB, IF, ICC, IP, ELISA, IHC
Spezies Reaktivität: Human
Immunogen: Human SOD1 8-14 amino acids.
Konjugation: IgG2b
Alternative Synonym: Superoxide Dismutase 1,Als,Als1,Hel-S-44,Ipoa,Sod,Hsod1,Homodimer,Sod1
Klonalität: Monoclonal
Konzentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: MS785
Molekulargewicht: 16
Isotyp: IgG2b
NCBI: 6647
Puffer: 1x PBS, 50% glycerol
Reinheit: Protein G Purified.
Formulierung: Liquid
Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4-8ºC. For long-term storage, aliquot and store at -20-8ºC or below. Avoid multiple freeze-thaw cycles.
Anwendungsbeschreibung: ICC/IF: 1 μg/ml (1/500 dilution) -5 μg/ml (1/100 dilution).Target : SOD1 mutants.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications., IP: 1-5μg/sample.Target : SOD1 mutants under native condition ,...
 IP analysis of HEK293 cells cultured in the presence and absence of 10μM TPEN (a potent zinc-specific chelator, for 8 hours) using GTX57211 SOD1 (mutant) antibody [MS785]. Under the zinc-deficient ER-stress, these antibodies could recognize endogenous SOD1 wild type with mutant like conformation. Antibody amount : 1-5 μg
 IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibody’s epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in “Appendix”. Antibody amount : MS27 (GTX57212): 2μg, MS785 (GTX57211): 5μg, or MS27/MS785 cocktail: 1μg IP lysis buffer: 1% Triton X100/TBS buffer.
 ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.2% Triton X-100 for 5 min Dilution : 1μg/ml (12 hours at 4℃)