SOD1 (mutant) antibody [MS785], IgG2b, Unconjugated, Rat, Monoclonal

Artikelnummer: GTX-GTX57211
Artikelname: SOD1 (mutant) antibody [MS785], IgG2b, Unconjugated, Rat, Monoclonal
Artikelnummer: GTX-GTX57211
Hersteller Artikelnummer: GTX57211
Alternativnummer: GTX57211-200
Hersteller: Genetex
Wirt: Rat
Kategorie: Antikörper
Applikation: WB, ICC/IF, IP, ELISA, IHC
Spezies Reaktivität: Human
Immunogen: Human SOD1 8-14 amino acids.
Konjugation: Unconjugated
Alternative Synonym: ALS, ALS1, Cu/Zn superoxide dismutase, epididymis secretory protein Li 44, HEL-S-44, homodimer, hSod1, indophenoloxidase A, IPOA, SOD, SOD, soluble, superoxide dismutase [Cu-Zn], superoxide dismutase 1, soluble, superoxide dismutase, cystolic
Klonalität: Monoclonal
Konzentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: MS785
Isotyp: IgG2b
NCBI: 6647
Puffer: 1x PBS, 50% glycerol
Reinheit: Protein G Purified.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: superoxide dismutase 1
Anwendungsbeschreibung: WB: Assay dependent. ICC/IF: 1 µg/ml (1/500 dilution) -5 µg/ml (1/100 dilution). IP: 1-5µg/sample. ELISA: Assay dependent. IHC: 1 µg/ml (1/500 dilution) -5 µg/ml (1/100 dilution). Target : SOD1 mutants. Target : SOD1 mutants under native condition ; Wild-type SOD1 under the zinc-deficient ER-stress. GTX57211 and GTX57212 could recognize not only mutants but also denatured SOD1. Please keep non-denatured condition and avoid to use high concentration of SDS or the other denaturing compounds.. Target : SOD1 mutants. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
 ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail.
Fixation : 4% PFA for 10 min at RT.
Permeabilization : 0.2% Triton X-100 for 5 min
Dilution : 1μg/ml (12 hours at 4ºC)
 IP analysis of HEK293 cells cultured in the presence and absence of 10μM TPEN (a potent zinc-specific chelator, for 8 hours) using GTX57211 SOD1 (mutant) antibody [MS785]. Under the zinc-deficient ER-stress, these antibodies could recognize endogenous SOD1 wild type with mutant like conformation.
Antibody amount : 1-5 μg
 IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibody’s epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in “Appendix”.
Antibody amount :
MS27 (GTX57212): 2μg, MS785 (GTX57211): 5μg, or MS27/MS785 cocktail: 1μg
IP lysis buffer: 1% Triton X100/TBS buffer.