Recombinant PARP Monoclonal Antibody (ET1608-56)

Artikelnummer: HBO-ET1608-56TR
Artikelname: Recombinant PARP Monoclonal Antibody (ET1608-56)
Artikelnummer: HBO-ET1608-56TR
Hersteller Artikelnummer: ET1608-56TR
Alternativnummer: HBO-ET1608-56TR
Hersteller: HUABIO
Kategorie: Antikörper
Trial Size
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IF, IHC-P, FC, WB
Spezies Reaktivität: Human, Rat, Mouse
Immunogen: recombinant protein
Konjugation: unconjugated
Alternative Synonym: ADP ribosyltransferase (NAD+, poly (ADP ribose) polymerase) antibody, ADP ribosyltransferase diphtheria toxin like 1 antibody, ADP ribosyltransferase NAD(+) antibody, ADPRT 1 antibody, ADPRT antibody, ADPRT1 antibody, ARTD1 antibody, msPARP antibody, NAD
Klonalität: Monoclonal
Konzentration: 1 ug/ul
Klon-Bezeichnung: SU03-68
Molekulargewicht: 113/89 kDa
Isotyp: IgG
UniProt: P09874
Puffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Reinheit: Protein A affinity purified.
Target-Kategorie: PARP

TARGET

UNIPROT #P09874

PROTEIN NAME PARP

SYNONYMS ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody; ADP ribosyltransferase diphtheria toxin like 1 antibody; ADP ribosyltransferase NAD(+) antibody; ADPRT 1 antibody; ADPRT antibody; ADPRT1 antibody; ARTD1 antibody; msPARP antibody; NAD(+) ADP ribosyltransferase 1 antibody; NAD(+) ADP-ribosyltransferase 1 antibody; pADPRT 1 antibody; pADPRT1 antibody; PARP 1 antibody; PARP antibody; PARP-1 antibody; PARP1 antibody; PARP1_HUMAN antibody; Poly (ADP ribose) polymerase 1 antibody; poly (ADP ribose) polymerase family, member 1 antibody; Poly [ADP-ribose] polymerase 1 antibody; Poly(ADP ribose) polymerase antibody; poly(ADP ribose) synthetase antibody; poly(ADP ribosyl)transferase antibody; Poly[ADP ribose] synthetase 1 antibody; Poly[ADP-ribose] synthase 1 antibody; PPOL antibody

POST-TRANSLATIONAL MODIFICATION Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.

SUBCELLULAR LOCATION Nucleus.

FUNCTION PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

Klonalität: Monoclonal
Tag: Trial Size
Antibody Type: Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.