ICAM5 Rabbit mAb, Unconjugated
Artikelnummer:
ABB-A26618
- Bilder (7)
| Artikelname: | ICAM5 Rabbit mAb, Unconjugated |
| Artikelnummer: | ABB-A26618 |
| Hersteller Artikelnummer: | A26618 |
| Alternativnummer: | ABB-A26618-20UL, ABB-A26618-100UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-P, WB |
| Spezies Reaktivität: | Mouse |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | TLN, CD50, Tlcn, Icam3, ICAM-3, ICAM-5 |
| Predicted to enable integrin binding activity. Acts upstream of or within phagocytosis. Located in plasma membrane. Is expressed in brain. Orthologous to human ICAM5 (intercellular adhesion molecule 5). |
| Application Verdünnung: | WB,1:50000 - 1:300000|IF-P,1:400 - 1:1600|IHC-P,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat |
|
|
Western blot analysis of various lysates using ICAM5 Rabbit mAb (A26618) at 1:50000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
|
|
Immunohistochemistry analysis of paraffin-embedded Human brain tissue using ICAM5 Rabbit mAb (A26618) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
|
|
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using ICAM5 Rabbit mAb (A26618) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
|
|
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using ICAM5 Rabbit mAb (A26618) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
|
|
Confocal imaging of paraffin-embedded Human brain tissue using ICAM5 Rabbit mAb (A26618, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x. |
|
|
Confocal imaging of paraffin-embedded Rat brain tissue using ICAM5 Rabbit mAb (A26618, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x. |
|
|
Confocal imaging of paraffin-embedded Mouse brain tissue using ICAM5 Rabbit mAb (A26618, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x. |
Produktgarantie und fachkundiger Support
