Goat IgG anti-Rabbit IgG (Fc)-unconj., MinX Hu, Polyclonal , Unconjugated

Product Overview

• Article Name: Goat IgG anti-Rabbit IgG (Fc)-unconj., MinX Hu, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-611-1123
• Supplier Catalog Number: 611-1123
• Alternative Catalog Number: ROC-611-1123
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Anti Rabbit IgG F(c) was produced by repeated immunization with rabbit IgG F(c) fragment in goat.
• Conjugation: Unconjugated
• Alternative Names: goat anti-Rabbit F(c) Antibody, Gt-a-Rabbit IgG F(c), Rabbit Antibody in Goat, Goat anti-Rabbit F(c) fragment Secondary Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 2mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Rabbit IgG F(c) is tested by immunoelectrophoresis, ELISA, dot blot, and western blot. This product is suitable for use in western-blot, competitive western-blot, and competitive ELISA assays. Specific conditions for reactivity and signal detection

Images

ELISA results of purified Goat Anti-Rabbit IgG F(c) Antibody (Min x Human serum proteins) tested against purified Rabbit IgG F(c) (Red Line), Rabbit IgG (Green Line), and Human IgG (Blue Line). Each well was coated in duplicate with 1.0 µg of antigen Rabbit IgG F(c) (011-0103), Rabbit IgG (011-0102), and Human IgG (009-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Donkey Anti-Goat IgG HRP (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).

Dot Blot Results of Goat Anti-Rabbit F(c) mx Human IgG. Row A: Rabbit F(c) protein (p/n 011-0103). Row B: Rabbit F(ab) protein (p/n 011-0105). Row C: Human IgG protein (009-0102). Amount Dotted: (1) 10ng, (2) 3.33ng, (3) 1.11ng, (4) 0.37ng, (5) 0.12ng. Primary Antibody: Anti-Rabbit F(c) at 1.0µL/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG Peroxidase Conjugate at 1:40,000 for 30mins at RT. Block: BlockOut Buffer (p/n MB-073). Exposure: 60secs.

Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

Dot Blot of Goat Anti-RABBIT IgG F(c) Antibody (Min X Human Serum Proteins). Row A: Rabbit IgG whole Molecule (p/n 011-0102). Row B: Rabbit F(c) (p/n 011-0103). Row C, Rabbit F(ab) (p/n -011-0105). Samples Loaded: 1- 100ng, 2- 33.33ng, 3- 11.11ng, 4- 3.70ng, 5- 1.23ng. Primary Antibody: Goat Anti-RABBIT IgG F(c) Mx Hu at 1.0µg/mL for 60mins at RT. Secondary Antibody: Custom HRP at 1:40,000 for 30mins at RT. Blocking (p/n MB-070) for 60 mins at RT. Exposure: 5 sec.

Western Blot of Goat anti-Rabbit Fc antibody. Lane 1: Rabbit Fc (p/n 011-0103). Load: 100 ng per lane. Primary antibody: Rabbit Fc antibody at 1:1,000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Rabbit Fc. Other band(s): Rabbit Fc splice variants and isoforms.