0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
Application Notes:
F(ab)2 Anti-Mouse IgG (H&L) Phycoerythrin Conjugated Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low
Dot Blot of Phycoerythrin conjugated Goat F(ab)2 Anti Mouse IgG (H&L) Antibody. Lane 1: Mouse IgG. Load: A three-fold serial dilution starting at 200ng. Primary Antibody: None. Secondary Antibody: F(ab)2 Anti-Mouse IgG (H&L) (GOAT) Antibody Phycoerythrin conjugated secondary antibody was used at 1:1000 for 1 Hour at RT. Blocking: 5% Blotto for 1 Hour at 20C.
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