Description
The Vector Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.
Features:
- Greater sensitivity than conventional substrates
- Consistent and reliable
- Ideal for IHC/ICC/ISH and blots
- Fluorescent
- Heat Stable
- Permanent mounting
- Stock solutions supplied in convenient dropper bottles promoting ease of handling
- No wait times for mixing and dissolving powders or tablets
- Sufficient reagents to produce 200 ml of working solution
- Excitation: broad, peak at ~500 nm*
- Emission: broad, peak at ~680 nm*
*Histochem J. (1998) 8:577
Specifications
| Unit Size |
1 kit |
| Applications |
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) |
Alkaline Phosphatase |
| Substrate Reaction Product |
Precipitating Reaction Product |
| Chromogen Color |
Blue |
| Color of Fluorescence |
Far Red |
| Microscopy |
Brightfield, Fluorescence, Spectral Imaging |
| Additional Substrate Properties |
Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling |
| Mounting |
Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent) |
Kit Components
Kit Contents:
- 4 ml Vector Blue Reagent 1
- 4 ml Vector Blue Reagent 2
- 3 ml Vector Blue Reagent 3
